Barcoding workshop at the DZMB in Wilhelmshaven 13 – 18 May, 2008 

Barcoding of deep-sea organisms to establish a molecular library has become a real possibility with deep-sea organisms arriving in excellent condition from recent CeDAMar expeditions. The workshop was hosted by the German Centre for Marine Biodiversity Research (DZMB) to familiarise a wider audience with the methodology and facilitate practical work and to establish contacts with leading laboratories from the United States and Canada. A day of discussion allowed the participants to explore how CeDAMar can benefit from barcoding and how, in turn, large databases such as MarBOL can grow with the help of CeDAMar (click here to download the discussion notes as PDF). 

The first day was dedicated to general introductions to the methodology, presentations of the leading laboratories, data bases, and the role of barcoding for CeDAMar. During the second day, practical lab work and introduction to work with barcoding databases (BoLD) was the focus, followed by the theoretical background on the third day. Day four was used for discussion and presentation of barcoding projects already running. Publishing outgoing cruises and the material available for barcoding on the CeDAMar website was agreed on. CeDAMar is also going to provide data to BoLD.  

Program Day 1 (Tuesday, 13th)

14:15 h: Welcome (S. Brix, K. Meißner)

14.30 – 15:00 h: Molecular approaches in CeDAMar (P. Martinez, G. Paterson)

15.00 – 15.30 h: CBOL leading labs presentations (A. Driskell, D. Steinke)

15:30 – 16:00 h: Marine Barcode of Life (MarBoL, D. Steinke)

16:30 – 17:30 h: Methods for new Barcoders (A. Driskell)  

Day 2 (Wednesday, 14th)

8:30 – 9:00 h: Barcoding, taxonomy and systematics – CBOL (Narration by D. Schindel)

 9:00 – 12:30 h: Practical demonstration lab work (A. Driskell)

14:00 – 15:00 h: Introduction to BoLD – Barcode of Life Database (D. Steinke)

15:00 – 15:30 h: The CAML Barcoding Initiative (R. Grant)

16:00 – 17:30 h: Practical training in BoLD (D. Steinke) 

Day 3 (Thursday, 15th)

9:00 – 10:30 h: Pros and Cons of DNA barcoding - a theoretical background (C. Schander)

11:00 – 11.30 h: Barcoding applications 1: Chip technology (M. Kochzius)

11:30 – 12:30 h: Barcoding applications 2: Phylogeography, e.g. networking (M. Raupach)

14.00 – 15:30 h: Barcoding applications 3: Phylogenetic approaches (C. Bleidorn)

16:00 – 17.30 h: Time for practical work (A. Driskell)  

Day 4 (Friday, 16th)

9:00 – 10:30 h: Foraminiferans – current molecular research (J. Pawlowski)

10:30 – 11:00 h: The Sponge Barcoding Project (G. Wörheide)

11.30 – 12.30 h: Discussion - Status quo and future steps

14.00 – 16:30 h: Discussion - Outcome for the CeDAMar community