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    MARBEF meiobenthos workshop 2007MARBEF meiobenthos workshop 2007

MARBEF meiobenthos workshop 2007

 

Marbef-MANUELA Meiobenthos Workshop, 15 - 20 October, 2007, DZMB, Wilhelmshaven

Report

 

 

1. Introduction

 

The workshop was hosted by DZMB in Wilhelmshaven and was organized by Pedro Martinez, Gritta Veit-Köhler and Lena Menzel. The aim of the workshop was to demonstrate the different techniques that are used within Europe. This techniques range from sampling; sampling processing and an introduction to the identification of the dominant meiobenthic taxa: the nematodes and the copepods.

 

The workshop was attended by 16 participants from 6 countries within and outside Europe. Not all participants were meiobenthologists, research fields of the participants included macrobenthos and protests. Most of the participants were active in science conducted in institutes or universities but it should be noted that one participant represented an SME.

 

2. Programme

 

Since the aim of the programme was to demonstrate all possible techniques that were used in Europe, a fixed programme could not be followed. This is due to the timing of the different techniques: eg. transforming nematodes from formalin samples to glycerol slides takes three days using the methods applied in Ghent, while it takes only 1 night following the UK procedure. Therefore it was not possible to stick to a fixed scheme. However the timing of the different techniques made it possible for all participants to (1) get to know the different techniques and (2) to practice the different techniques. By Thursday afternoon (18 October 2007), all techniques were done and slides for nematodes were made. This was followed by a session aiming at identification of nematodes. Friday (19 October 2007) morning was devoted to copepod identification while the afternoon was spent on a lecture on stable isotope techniques in meiobenthic research.

 

3. Workshop content

 

The first aim of the workshop to inform the participants about different methods of sampling, both at sea and in the intertidal zone. This was done by dr. Michaela Schratzberger (CEFAS, UK) and Dr. Jan Vanaverbeke (Ghent University, Belgium). During a tour trough the institure, dr. Pedro Martinez, explained the way the different gear,discussed in the theoretical part, have to be operated.

 

Following this, an excursion was made to the intertidal area in front of the institute to collect sufficient samples to be processed during the rest of the workshop. Samples were taken in muddy and fine sandy environments using different core sizes. Extraction of meiobenthos from the sediment was done using both the flotation technique and the centrifugation technique. For both methods, small differences exist and all of them were shown and explained using the information sent to the organizers by various MANUELA partners. Slicing of sediments using different methods was shown as well. Workshop participants were expected to take active part in the processing of samples. Dr. Andre Esteves (Universidade Federal de Pernambuco, Brasil) kindly explained alternative techniques in sample processing widely used in Brasil.

 

When fauna was extracted, samples were sorted and nematodes and copepods where picked in order to transfer them to slides. Again, different methods of this protocol were explained and practiced by the participants. Subsampling procedures were explained and shown both in a theoretical lecture (Michaela Schratzberger and Jan Vanaverbeke) and shown in the lab. Slide making for nematodes was shown by Michaela Schratzberger en Bart Beuselinck (Ghent University) and included the making of “whole sample” mounts, smaller slides and Cobb slides. Gritta Veit-Köhler and Lena Menzel showed the different ways of storing harpacticoid specimens and slide making for various partners. Again, participants were encouraged to make all kind of slides themselves.An introduction to the identification of marine nematodes using the series of pictorial keys of Platt and Warwick and the online identification system NeMys was given by

 

Michaela Schratzberger and Jan Vanaverbeke) and shown in the lab. Slide making for nematodes was shown by Michaela Schratzberger en Bart Beuselinck (Ghent University) and included the making of “whole sample” mounts, smaller slides and Cobb slides. Gritta Veit-Köhler and Lena Menzel showed the different ways of storing harpacticoid specimens and slide making for various partners. Again, participants were encouraged to make all kind of slides themselves.An introduction to the identification of marine nematodes using the series of pictorial keys of Platt and Warwick and the online identification system NeMys was given by Jan Vanaverbeke. After this introduction, participants were invited to try to identify nematodes to the genus level. Practical assistance was given by Michaela Schratzberger and Jan Vanaverbeke. Gritta Veit-Köhler introduced the methods to identify harpacticoid copepods. After this, Lena Menzel, Gritta Veit-Köhler and Gisella Moura helped the participants with the identification of the harpacticoids present in the sample. To finish, dr. Tom Moens (Ghent University) gave a lecture on stable isotope techniques in meiobenthic research and focused on the practical methods, pitfalls and solutions to frequently occurring problems in this field of research.

 

Michaela Schratzberger and Jan Vanaverbeke) and shown in the lab. Slide making for nematodes was shown by Michaela Schratzberger en Bart Beuselinck (Ghent University) and included the making of “whole sample” mounts, smaller slides and Cobb slides. Gritta Veit-Köhler and Lena Menzel showed the different ways of storing harpacticoid specimens and slide making for various partners. Again, participants were encouraged to make all kind of slides themselves.An introduction to the identification of marine nematodes using the series of pictorial keys of Platt and Warwick and the online identification system NeMys was given by Jan Vanaverbeke. After this introduction, participants were invited to try to identify nematodes to the genus level. Practical assistance was given by Michaela Schratzberger and Jan Vanaverbeke. Gritta Veit-Köhler introduced the methods to identify harpacticoid copepods. After this, Lena Menzel, Gritta Veit-Köhler and Gisella Moura helped the participants with the identification of the harpacticoids present in the sample. To finish, dr. Tom Moens (Ghent University) gave a lecture on stable isotope techniques in meiobenthic research and focused on the practical methods, pitfalls and solutions to frequently occurring problems in this field of research.

 

All participants warmly acknowledge the hospitality of the DZMB institute and Gritta Veit-Köhler and Lena Menzel for the warm and enthusiastic way in which they organized the workshop.

 

4. Participants list

 

Adriane Wandeness, Universidade Federal de Pernambuco, Dept. Zoologia Brazil, wandenes@biologia.ufrj.br

 

Dr. André Morgado Esteves, Universidade Federal de Pernambuco, Dept. Zoologia Brazil, andreesteves@ufpe.br, esteves@biologia.ufrj.br

 

Annika Henche Forschungsinstitut Senckenberg, DZMB, Wilhelmshaven, Germany, ahenche@senckenberg.de

 

ahenche@senckenberg.de

 

Bart Beuselinck Marine Biology Section, Ghent University, Belgium, Bart.Beuselinck@ugent.be

 

Dr. Dror Angel Recanati Institute for Maritime Studies, Haifa University, Israel, adror@research.haifa.ac.il

 

Dr. Gritta Veit-Köhler, Forschungsinstitut Senckenberg, DZMB, Wilhelmshaven, Germany, gveit-koehler@senckenberg.de

 

Dr. Jan Vanaverbeke, Marine Biology Section, Ghent University, Belgium, jan.vanaverbeke@ugent.be

 

Jutta Heitfeld, Forschungsinstitut Senckenberg, DZMB, Wilhelmshaven, Germany,   jheitfeld@senckenberg.de

 

jheitfeld@senckenberg.de

 

Kasia Grzelak, Marine Ecology Department of the Institiute of Oceanology, Polish Academy of Scieces, Poland, kasiut7@wp.pl

 

Lena Menzel, Forschungsinstitut Senckenberg, DZMB, Wilhelmshaven, Germany, lmenzel@senckenberg.de

 

Dr. Michaela Schratzberger, CEFAS Laboratory, United Kingdom, michaela.schratzberger@cefas.co.uk

 

Prof. Dr. Pedro Martínez Arbizu, Forschungsinstitut Senckenberg, DZMB, Wilhelmshaven, Germany, pmartinez@senckenberg.de

 

Sarah Dashfield, Plymouth Marine Laboratory, United Kingdom, SLDAS@pml.ac.uk

 

Dr. Stephen Jarvis, Marine Invertebrate Ecological Services, United Kingdom, steve.jarvis@ic24.net

 

Tatiana Fabricio Maria, Universidade Federal de Pernambuco, Dept. Zoologia, Brazil, tatiana_fabricio@yahoo.com.br

 

Prof. Dr. Tom Moens, Marine Biology Section, Ghent University, Belgium, tom.moens@ugent.be

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